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Extension-nodules.jpg Texas A&M AgriLife
Sliced Bradyrhizobium nodules on the peanut plant demonstrate pink and red color indicating active fixation of nitrogen from the air.

Peanut growers encouraged to scout nodules

Peanut specialists discuss nodulation assessments and nitrogen applications.

Now is the time for peanut growers on the Texas South Plains to scout their crops for taproot Bradyrhizobium nodulation, says Dr. Emi Kimura, Extension Agronomy/State Extension peanut specialist, Texas A&M University Department of Soil & Crop Sciences, Vernon, in the June 13-issue of Texas Row Crops newsletter.

Scouting five to six weeks after planting assesses early nodulation in advance of possible decisions about applying mid-season nitrogen,” says Kimura. "If nitrogen is needed, this early assessment will help determine how much."

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When it comes to legumes, growers may not think about applying a nitrogen fertilizer, but if a grower recognizes soon enough he or she has few or no active nodules on the roots, nitrogen applications are almost certain, says Dr. Calvin Trostle, Extension Agronomy, TAMU Department of Soil & Crop Sciences, Lubbock. This applies to legumes such as peanuts, soybeans, peas and beans, guar and alfalfa.

While legumes are a valuable asset in any Texas crop rotation, Trostle says they also offer significant potential nitrogen fixation to the existing crop with the possibility of improving the soil nitrogen status for the subsequent crop.

But there is a caveat. “Legume and nitrogen-fixation benefits can only occur if the roots are infected by a crop-specific bacterial strain of either Rhizobium or Bradyrhizobium, which is either applied or may already be present in the soil,” he says.

And root bacterial infection leads to nodulation. “The bacteria survive and thrive inside these root nodules, visibly showing a pink or red color. That means fixation of nitrogen in the air is converted into a form the plants can use. This is the least costly form of nitrogen you can apply to your legume crop,” he says.


Application of an inoculant to legume seed at planting with little to no nodulation later on in the plant growth raises concerns, says Trostle. “First, you are not getting the nitrogen nutrition you may have expected for your crop, and therefore, fertilizer nitrogen may be needed to obtain your yield potential. Second, if you have no nodules, then we need to understand why. Was it the wrong inoculant, expired inoculant, improper application or was the inoculant introduced into hot, dry soils where the bacteria did not survive long enough to infect the roots?

To assess the taproot, rather than pull up the peanut plant and risk stripping off the nodules, Trostle recommends using a shovel but also selecting plants from different rows and field locations.

The ideal situation is when you also have a few rows with no inoculant applied to the seed, then you can truly see, if and how much, nodulation benefit you may have. For any legume crop, inoculated or not, if nodulation is deemed poor, nothing can be done to increase nodulation in the current crop,” says Trostle.

On the Texas South Plains, 20 to 25 percent of fields may be undernodulated, or worse, have only a few nodules per plant, says Trostle.  “Poor Bradyrhizobium nodulation calls for supplemental nitrogen to achieve desired yield potential, which is why early scouting is recommended. We need to know which fields are not nodulating early in the cropping season.”

While the peanut plants have lateral roots with nodules, suggesting a source of nodulation bacteria in the soil, Trostle says they should contrast to the masses of nodules found on the taproot, “evidence of inoculant application.” Lateral root nodules, though often high in number (hundreds per plant) tend to be less active and may not be fixing nitrogen for the peanut crop, he adds.

To determine whether a nodule is active, Trostle says the inside of it should be pink to dark red. “If nodules are white inside, they are not yet active and should be checked again in seven to 10 days.”

Nodules that are black, gray, and possibly mushy are no longer active. “This becomes common later in the cropping season even though the crop may still be growing. Applying late-season nitrogen after nodules slough off is not recommended,” says Trostle. 

Nodules that never turn pink or red inside are from soil Rhizobium/Bradyrhizobium that may not be specific for peanuts or other legume crops, he says. “You need to differentiate these types of nodules, mostly on the lateral roots, versus the mass of ‘super nodulation’ on the taproot, which is ready evidence that your inoculant worked.”


The rate chart below provides simple guidelines for early season Bradyrhizobium nodulation for peanuts on the Texas High Plains. “We are particularly interested in any developing clusters of nodules on the taproot,” says Trostle. “If early nodulation is good, you can expect it to continue to increase toward peak nodulation (about mid-August), but if early nodulation is poor, it probably isn’t going to improve.”



About mid-August to early September, Kimura recommends growers check for peak nodulation, which will confirm earlier observations. For peanuts or other legumes grown in other regions, this assessment should be done four to six weeks before physiological maturity, she says. 

When assessing, Kimura says growers should consider the following:

  1. Did nodulation improve?  "For peanuts, we hope for over 50 nodules per plant."
  2. If nodulation remains low, consider why it might not have occurred and what you can do to enhance nodulation in next year’s crop. (Consider flagging fields that nodulated poorly.)
  3. Do you see obvious signs where inoculant was misapplied?  "This could include individual yellow rows, but also spotty green/yellow color throughout the field or a light pea-green field color suggesting nitrogen deficiency."

Texas A&M AgriLifeExtension-peanuts-N-deficient.jpg

An inoculant application malfunction on row seven of an eight-row planter led to little or no inoculant application. Yellow peanuts show nitrogen deficiency. The August nodule count on this Hockley County, Texas, runner peanut field was nine nodules per plant in yellow rows and 73 nodules per plant on adjacent green rows.


While successful nodulation is expected after a grower inoculates, it is never assured, says Trostle. "Sometimes something goes wrong. A mass of Rhizobium or Bradyrhizobium nodules on the taproot is highly desirable, in contrast to large numbers of individual nodules spread randomly over the lateral roots."

Trostle lists the following major causes of minimal to no taproot nodulation, despite inoculant application:

  1. Poor placement of in-furrow granular or liquid inoculant. Make sure your liquid stream is coming right in on top of the seed or that granular drop hoses do likewise. Try to get your discharge point within 6 inches and less of the furrow between the disk openers. Use a Tee-jet or Stream-jet tip, not a fan tip, to better shoot liquid inoculant into the furrow. Be sure granular drop hoses flow freely and remain unclogged at the tip.  "Proper placement of inoculant in the furrow is probably more important than which inoculant you use."
  2. Shallow planting (<1.5 inches, especially <1.0 inches), particularly for liquid inoculants, where surface soil may become hot or dry out. Death of inoculum and reduced nodulation may also occur when little soil is drug back over the seed, even if planted 1.5 inches or deeper.
  3. In the Southwest, seedbox inoculant powders have shown little if any increased nodulation, let alone yield, versus uninoculated crops in most Texas A&M AgriLife trials, including peanut, black-eyed pea, soybean, and guar. "Though seedbox powders may be inexpensive, about $2 per acre, they are often largely ineffective due to low bacterial counts."
  4. Large amounts of starter nitrogen near the seed (or high residual soil nitrogen) at rates near 30 lbs. of nitrogen per acre or more, will curtail nodule development. "Large applications of mid-season nitrogen can reduce peak nodulation as peanut plants and other legumes take the lazy approach and use soil and fertilizer nitrogen before fostering nodule development."
  5. Low rates of inoculum."Calibrate granular and liquid inoculants to ensure adequate rates."

For more information about lesser factors which may explain poor legume crop nodulation or for more information about assessing nodulation in other Texas legumes, click here

For questions or more information, contact Kimura or Trostle

TAGS: Crops
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